Journal: Cell reports

Article Title: Inappropriate Intrusion of an Axonal Mitochondrial Anchor into Dendrites Causes Neurodegeneration

doi: 10.1016/j.celrep.2019.09.012

Figure Lengend Snippet: (A–F) Immunohistochemistry technique for capturing SNPH intrusion in PC dendrites. Shown is SNPH (green), Syt2 (red), and Calbindin (blue) labeling in 3.5-month-old WT (A, C, and E) and Shi (B, D, and F) mice. Scale bar, 10 μm. (G and H) High magnification of the maximum intensity projection image from the z stack through dendritic regions of WT (G) and Shi (H). (I and J) Orthogonal (slice) view of SNPH punctum (indicated by arrows in panels G and H) in the dendritic region from WT (I) and Shi (J) in x-z and y-z orientations, respectively. (K) Quantification of percentage area occupied by SNPH within the dendritic volume from 3 mice of each group. Data are shown as mean ± SEM. *p < 0.05. (L–N) Capturing SNPH intrusion by pre-tagging dendritic mitochondria in vivo using viral transduction. (L) Technique to selectively transduce PCs with AAV-Mito-mCherry. (M) Demonstration of successful pre-tagging of mitochondria in dendrites of a single PC by Calbindin staining. Scale bar, 10 μm. (N) Demonstration of how pre-tagged dendritic mitochondria in PCs are used to capture SNPH intrusion by 3D rotation. (O and P) Single dendritic tree in WT (O) or Shi (P) pre-tagged with Mito-mCherry (red) and SNPH intrusion (green) captured by co-rotation with Mito-mCherry. Merged images show the fraction of dendritic mitochondria anchored by intruded SNPH (yellow). (Q and R) Percentage of PCs with SNPH intrusions (Q) and percentage of SNPH bound to mitochondria per dendritic tree (R) from WT (n = 73) and Shi (n = 106) PCs. Data are shown as mean ± SEM. ***p < 0.005.

Article Snippet: Polyclonal Calbindin , Boster , M03047–2.

Techniques: Immunohistochemistry, Labeling, In Vivo, Transduction, Staining

Journal: Cell reports

Article Title: Inappropriate Intrusion of an Axonal Mitochondrial Anchor into Dendrites Causes Neurodegeneration

doi: 10.1016/j.celrep.2019.09.012

Figure Lengend Snippet: (A and B) Representative images of lentivirally transduced GFP-SNPH (1–469) (A) and GFP-SNPH (B) in PCs of SNPH-KO mice injected with saline (no harmaline, vehicle only). (C-H) Effect of harmaline on GFP-SNPH (1–469)-transduced (C) and GFP-SNPH-transduced (F) PC dendrites. Degenerating dendrites in GFP-SNPH-transduced PCs can be seen in (F). Also shown is Calbindin labeling of GFP SNPH (1–469) (D) and GFP-SNPH (G) from (C) and (F). Merged images of GFP SNPH (1–469) and GFP-SNPH with Calbindin are shown in (E) and (H), respectively. (I–K) Representative image of a harmaline-induced degenerating PC (white arrow in I) transduced with GFP-SNPH. Calbindin staining from the same section is shown in (J), whereas a merged image is shown in (K). Scale bars, 20 μm. (L) Quantification of dendritic shrinkage in GFP-SNPH (1–469)- and GFP-SNPH-transduced PCs in the absence (n = 3 mice, vehicle only) or presence of harmaline (n = 5 mice). Data are shown as mean ± SEM. ***p < 0.001.

Article Snippet: Polyclonal Calbindin , Boster , M03047–2.

Techniques: Injection, Saline, Labeling, Transduction, Staining

Journal: Cell reports

Article Title: Inappropriate Intrusion of an Axonal Mitochondrial Anchor into Dendrites Causes Neurodegeneration

doi: 10.1016/j.celrep.2019.09.012

Figure Lengend Snippet:

Article Snippet: Polyclonal Calbindin , Boster , M03047–2.

Techniques: Virus, Plasmid Preparation, Recombinant, Software, Imaging

Journal: eLife

Article Title: LKB1 coordinates neurite remodeling to drive synapse layer emergence in the outer retina

doi: 10.7554/eLife.56931

Figure Lengend Snippet: Antibodies used in LKB1 mutant tissue analysis. Antibodies were utilized that label individual neuron populations and synapses in the outer retina.

Article Snippet: Calbindin D-28k , Full-length recombinant human Calbindin D-28K , Horizontal cells; amacrine cells; retinal ganglion cells , Novus biologicals; chicken polyclonal; NBP2-50028; no RRID , 1:2000.

Techniques: Mutagenesis, Labeling, Concentration Assay, Recombinant, Derivative Assay, Purification

Journal: Scientific Reports

Article Title: The temporal progression of retinal degeneration and early-stage idebenone treatment in the Pde6b rd1/rd1 mouse model of retinal dystrophy

doi: 10.1038/s41598-024-52391-y

Figure Lengend Snippet: Antibodies utilized for immunohistochemical and immunofluorescent staining.

Article Snippet: Calbindin D28K , Novus , 1:1000/1:1000 , Chicken , NBP2-50028.

Techniques: Immunohistochemical staining, Staining

Journal: PLoS ONE

Article Title: Cold Shock Proteins Are Expressed in the Retina Following Exposure to Low Temperatures

doi: 10.1371/journal.pone.0161458

Figure Lengend Snippet: Primary and secondary antibodies used in this study.

Article Snippet: Calbindin D28K , Rabbit polyclonal , 1:150 , Santa Cruz Biotech. , sc-7691.

Techniques:

Journal: PLoS ONE

Article Title: Cold Shock Proteins Are Expressed in the Retina Following Exposure to Low Temperatures

doi: 10.1371/journal.pone.0161458

Figure Lengend Snippet: Representative confocal microscopy images of colocalizations in hypothermic adult rat retina between CIRP (Red, A,D,G) and cell specific markers calbindin (B), glutamine synthetase (E), and recoverin (H). The third column is a combination of the first two; a yellow hue represents colocalization. GCL = ganglion cell layer, IPL = inner plexiform layer, INL = inner nuclear layer, OPL = outer plexiform layer, ONL = outer nuclear layer. Bar for A-C = 50 μm. Bar for D-F = 100 μm. Bar for G-I = 50 μm.

Article Snippet: Calbindin D28K , Rabbit polyclonal , 1:150 , Santa Cruz Biotech. , sc-7691.

Techniques: Confocal Microscopy

Journal: PLoS ONE

Article Title: Cold Shock Proteins Are Expressed in the Retina Following Exposure to Low Temperatures

doi: 10.1371/journal.pone.0161458

Figure Lengend Snippet: Representative confocal microscopy images of colocalizations in hypothermic adult rat retina between RBM3 (A,E,G) and cell specific markers calbindin (B), glutamine synthetase (D), and recoverin (H). The third column is a combination of the first two; a yellow hue represents colocalization. GCL = ganglion cell layer, IPL = inner plexiform layer, INL = inner nuclear layer, OPL = outer plexiform layer, ONL = outer nuclear layer. Bar for A-C = 50 μm. Bar for D-F = 25 μm. Bar for G-I = 25 μm.

Article Snippet: Calbindin D28K , Rabbit polyclonal , 1:150 , Santa Cruz Biotech. , sc-7691.

Techniques: Confocal Microscopy